ABSTRACT
Schistosoma mansoni infection induces in their hosts a marked and sustained eosinophilia, which is influenced or modulated by complex mechanisms, that vary according to the phase of infection. To address this phenomenon, we used the air pouch (AP) model in control and infected Swiss webster mice, analyzing the cellular, tissue response and local expression of adhesion molecules [CD18 (ß2-chain), CD44, ICAM-1 (CD54), L-selectin (CD62L), CD49d (alpha4-chain), LFA1 (CD11a)]. Infected animals were studied at 3 (pre-oviposition phase), 7 (acute phase), and 14 (chronic phase) weeks after infection (5-6 mice/period of infection). Normal mice were age-matched. Results showed that after egg stimulation, compared with matched controls, the infected mice, at each point of infection, showed a lower eosinophil response in the acute (7 weeks) and chronic phase (14 weeks) of infection. However, when the infected mice were in pre-oviposition phase (3 weeks) their eosinophil response surpassed the control ones. In the AP wall of infected mice, a significant decrease in the expression of ICAM-1 and CD44 in fibroblastic-like cells and a reduction in the number of CD18 and CD11 a in migratory cells were observed. The other adhesion molecules were negative or weakly expressed. The results indicated that in the air pouch model, in S. mansoni-infected mice: (1) eosinophil response is strikingly down-regulated, during the acute ovular phase; (2) in the pre-oviposition phase, in contrast, it occurs as up-regulatory modulation of eosinophil responses, in which the mechanisms are completely unknown; (3) in the chronic phase of the infection, the down modulation of eosinophil response is less pronounced; (4) Down-regulation of adhesion molecules, specially of ICAM-1 appear to be associated with the lower eosinophil response.
Subject(s)
Animals , Mice , Eosinophilia/parasitology , Schistosoma mansoni/parasitology , Cell Adhesion Molecules , Infections/parasitologyABSTRACT
Thirteen clinicaly healthy crossbred (Holstein x Zebu) aged between 20 and 45 months, raised in Rio de Janeiro State, Brazil, were utilized. Samples of blood (2), without anticoagulant, were colected from each animal by jugular vein puncture. It was determined the values for total protein, albumin (commercial kits), globulins and albumin/globulins ratio by mathematics calculation. The observed limits, mean and respective standard deviation in g/dl were: 5.70 to 8.80 and 7.01 more or less 0.65 (total protein); 2.60 to 4.20 and 3.10 more or less 0.36 (albumin); 2.30 to 5.00 and 3.70 more or less 0.59 (globulins) and 0.60 to 3.50 and 2.25 more or less 0.87 (albumin/globulins ratio)
Subject(s)
Animals , Female , Cattle , Proteins/supply & distributionABSTRACT
Os autores compararam 14 pacientes portadores de dermato-polimiosite primaria (DP) com pacientes portadores de reumatismo tumoral nao invasivo (RTNI) e neoplasias malignas. As idades medias no DP, DS, RNTI e neoplasias malignas foram de 25,3 mais ou menos 19,0, 47,8 mais ou menos 10,8, 51,7 mais ou menos 14,1 e 58,2 mais ou menos 13,3 anos respectivamente.Houve preponderancia do sexo masculino no grupo RTNI e feminino do DS tumoral (DST). A terapeutica para a DST mostrou-se mais eficaz com o uso de quimioterapia, radioterapia do que com o uso de anti-inflamatorios. Os dados obtidos sugerem que as dermato-polimiosite tumorais possam surgir numa faixa etaria intermediaria entre a DP e tumores malignos. A incidencia de neoplasias em dermato-polimiosite foi de 28,5%, enquanto a incidencia de DST para uma populacao de portadores de RNTI e neoplasias foi respectivamente de 22,2% e 1,1%. Especula-se tambem a respeito de um possivel mecanismo imunopatologico da DST pelo qual o nucleo tumoral poderia ter a capacidade de desviar do tumor uma populacao de celulas capazes de reagir a antigenos tumorais de uma maneira tal que a reacao se daria a nivel do sistema muscular